Parameter Explanations ====================== Image Source ++++++++++++ ALICA offers 3 different options of acquiring images from MicroManager: - **MM Core** Images are drawn directly from the circular buffer. This method is the fastest, and recommended in most cases, since it can smoothly function whenever the camera is acquiring images. - **Live mode** Images are drawn from the Datastore associated with the current live mode view. Use this if you wish to do some on-the-fly processing using the MicroManager pipeline, before passing the image to Analyzer. - **Next acquisition** Images will be drawn from the Datastore associated with the first acquisition that is started afterwards. ROI +++ Using the live view, you can select a region of interest to constrain the analyzed area (for example if the density of fluorophores is uneven, or the analysis of full image takes too long). Controller tick rate ++++++++++++++++++++ This value in milliseconds defines how often the Controller queries the Analyzer, and adjusts the laser output. Laser +++++ - **Max Power** Maximal power setpoint of the laser. ALICA will not adjust the laser power above this value. - **Deadzone [%]** The minimum adjustment to the power setpoint that the controller may make as a percentage of the current value. Adjustments to the laser by an amount less than this are not permitted, which prevents unnecessary fine-tuning of the laser. - **Virtual** If checked, the output is not passed to the device. Useful for debugging or preview of parameters.